The Applied Biosystems Quantstudio Absolute Q is a plate-based digital PCR system that provides absolute quantification of nucleic acid target sequences. Unlike qPCR, dPCR does not rely on standard curves for quantification. It is more sensitive than qPCR and thus well-suited to tasks such as detecting rare alleles or determining copy number variance.
The Absolute Q plates can fit up to 16 samples at a time.
Visit the ThermoFisher website for more information.
A membership is required to access the BioGarage facility.
Training:
Before being given instrument access, users are required to attend a training session. To set up a training, please fill out training request below:
After training, instrument time can be booked using our online scheduling tool. A link to the scheduling tool can be found on the Scheduling page. The Absolute Q is available for use on weekdays from 8 am to 4 pm. See further policies regarding instrument usage and training below.
UWBC BioGarage Scheduling and Use Policy
Materials:
To run the instrument, you will need a plate kit and dPCR master mix. Individual plates can be purchased directly from the BioGarage for $150 each or you can order your own plate kits. Master mix cannot be purchased through the BioGarage and must be provided by the user. Catalog numbers for necessary materials are below.
Starter Kit (12 plates and master mix):
- QuantStudio Absolute Q MAP16 Plate Kit and Master Mix (Applied Biosystems A53301)
Plates only:
- QuantStudio Absolute Q MAP16 Plate Kit (Applied Biosystems, Catalog no: A52865)
Master mix only:
- Absolute Q DNA Digital PCR Master Mix (5x) (Applied Biosystems, Catalog no: A52490)
- Absolute Q 1-step RT-dPCR Master Mix (4X) (Applied Biosystems, Catalog no: A55146)
Guides:
Absolute Q Digital PCR System User Guide
Absolute Q Quick Start Guide
Pricing:
| UW System rate | Non-UW System rate | |
| Training Session | $150 | $232.50 |
| Usage fee (per run) | $50 | $77.50 |
| MAP16 Plate kit (per plate) | $150 | $232.50 |